Pichiapastoris相关论文
生物界中大量的蛋白质都是以前体分子的形式被合成的,主要由信号肽、前导肽和成熟肽三个部分组成,其中前导肽对成熟肽的正确折叠起......
Notch信号途径广泛存在于多种生物体内,且进化上高度保守,在胚胎发育、血细胞发育及肿瘤形成等多种病理生理过程中发挥重要作用。N......
WCAP2013-1-02-034 Gene cloning of porcine adiponectin gene from adipose tissue and construction of i
Adiponectin (ADPN) is a kind of active protein mainly secreted by adipose tissues,and was identified independently by fo......
The commonly used medium to cultivate recombinant Pichia patoris,BMGY and BMMY,are too costly to put into industrial pra......
A novel and robust recombinant Pichia pastoris yeast whole cell biocatalyst with intracellular overe
A novel and robust recombinant Pichia pastoris yeast whole cell catalyst(WCC)with functional intracellular expression of......
维持细胞中正常的脂质组成对于许多细胞过程是至关重要的。前期研究已经证实Spt23 是胞内重要的转录因子，调控脂肪酸脱氢酶基因的表......
The antimicrobial peptide Lacticin Q(LQ), which contains 53 amino acid residues, is a novel antimicrobial (AMPs) produce......
...
参照毕赤氏巴斯德酵母(Piehiapastoris)偏好密码子,改造并化学合成杂合抗菌肽cecA-mil基因,改造后的cecA-mil基因克隆到pPICZα-A......
Construction of recombinant yeast expressing Pre S2120-146 and HBsAg of Hepatitis B Virus and its im
Objective: To construct the recombinant yeast expressing Pre S212o-146 and HBsAg of Hepatitis B Virus, and evaluate its ......
Escherichia coli phytase appA belongs to the histidine acid phosphatase family.With a high hydrolytic activity towards p......
Pichia pastoris expression system is characterized with simple culture conditions,low nutrient requirements,clear geneti......
Characterization and high-yield production of non-N-glycosylated recombinant human BCMA-Fc in Pichia
B-cell maturation antigen(BCMA)fused at the C-terminus to the Fc portion of human IgG1(BCMA-Fc)blocks B-cellactivating f......
本研究采用流加补料培养方式培养重组Pichia pastoris,表达血管生长抑制素(Angiostatin).整个培养过程分为以甘油为碳源的生长阶段......
The Different Extron-intron Recombination of Horseradish Peroxidase Isozyme C2 Gene and Biological A
Objective: The different extron-intron recombination of horseradish peroxidase isozyme C2 (HRPC2) gene were constructed,......
To produce an effective vaccine to protect chickens against Proteus mirabilis infection, OmpA, one of the major outer me......
Expression of laccase gene from white rot fungus in Pichia pastoris can enhance the resistance of ye
Laccase is a copper-containing polyphenol oxidase mainly produced from white rot fungi.Although laccase has great potent......
Expression, purification and characterization ofa thmmbin-like enzyme from snake venom of Gloydius s
...
Porcine β-defensin 2 (pBD2), a recently discovered porcine defensin that is produced by the intestine, exerts antim......
EplT4 proteinaceous elicitor expression in Pichia pastoris as an indicator of plant induction in soy
A cDNA library was constructed from the mycelium of Trichoderma asperellum T4 and a highly expressed gene fragment n......
O20Expression of a Xylanase gene from the thermophilic Paecilomyces thermophila in Pichia pastoris a
...
Porcine β-defensin 2 (pBD2), a recently discovered porcine defensin that is produced by the intestine, exerts antim......
应用RTPCR方法扩增牛脂联素（Bovine adiponectin，BovADPN）基因，连接到载体pMD18-T中；测序并利用BLAST工具对比证明该基因序列正确，经EcoR......
为使IpaC基因表达产物保持天然的生物学活性,实现在酵母表达系统中高效表达,本研究首先采用反向PCR将IpaC基因358～360位和361～363位......
应用特异性引物,从鹅源副黏病毒NA-1株中扩增出HN蛋白基因,PCR产物纯化后克隆入pGEM-T载体,得到重组质粒pTHN。用EcoRⅠ和NotⅠ双......
High-yield expression of recombinant mouse coagulation factor Ⅶ in methylotrophic yeast Pichia pasto
Objective: To explore high-yield secretory expression of recombinant mouse coagulation factor Ⅶ (rmF Ⅶ ) protein in Pi......
Objective:To construct a Pichia pastoris (P. Pastoris) expression vector of human intestinal trefoil factor (hITF) and s......
目的 利用毕赤酵母诱导表达T4溶菌酶蛋白并测定其抗菌活性.方法 T4溶菌酶(T4 lysozyme)基因以N端融合的方式被准确插入到质粒pPIC9......
High-yield expression of recombinant SARS coronavirus nucleocapsid protein in methylotrophic yeast P
AIM: Nucleocapsid (N) protein plays an important role in reproduction and pathological reaction of severe acute respirat......
AIM: To construct a yeast expression system of human augmenter of liver regeneration (hALR) and to examine its bioactivi......
Secretory expression and characterization of a recombinant deleted variant of human hepatocyte growt
AIM: To study the secretory expression of human hepatocyte growth factor (hdHGF) gene in Pichia pastoris.METHODS: The fu......
Expression and Antigenic Characterization of the Epitope-G1 of the Bovine Ephemeral Fever Virus Glyc
The epitope-G1 gene of Bovine ephemeral fever virus (BEFV) glycoprotein was synthesised by PCR and cloned into expressio......
Expression of lignin peroxidase H2 from Phanerochaete chrysosporium by multi-copy recombinant Pichia
The lipH2 gene, encoding the expression of lignin peroxidase, was cloned from Phanerochaete chrysosporium BKM-F-1767 and......
Objective:To construct a Pichia pastoris (P. Pastoris) expression vector of human intestinal trefoil factor (hITF) and s......
High-yield expression of recombinant mouse coagulation factor Ⅶ in methylotrophic yeast Pichia pasto
Objective: To explore high-yield secretory expression of recombinant mouse coagulation factor Ⅶ (rmF Ⅶ ) protein in Pi......
β-Mannanase catalyzes endo-wise hydrolysis of the backbone of mannan and heteromannan, which are abun-dant in the cell ......
该文由两部分组成.第一部分,重组人血清白蛋白在Pichiapastoris中的表达.为获得高产稳产人血清白蛋白的Pichiapastoris细胞株用于......
唾液富组蛋白5(histidine-rich proteins 5，简写HRP 5)，为一直链阳离子多肽，由24个氨基酸(AA)组成，含7个组氨酸，分子量3037Da，主要存......
在摇瓶水平上,采用单因素试验方法,对影响碱性果胶酯基因工程菌Pichia pastoris表达的主要因素进行研究.确定初始甲醇浓度、甲醇补......
The phyAm gene encoding acid phytase and optimized neutral phytase phyCs gene were inserted into expression vector pPIC9......
果糖基转移酶（EC 2.4.1.9）是利用蔗糖为底物制备低聚果糖的关键酶。利用前期构建的产果糖基转移酶的重组毕赤酵母为出发菌株,对其进......
为评价禽网状内皮组织增生病病毒(REV) env蛋白的免疫原性,本研究将env基因克隆至毕赤酵母表达载体pAO815,构建了含有两个env表达......
从大蒜鳞茎中克隆蒜酶基因,构建蒜酶真核表达载体,并在毕赤酵母系统中诱导表达,探讨重组蒜酶的活性及其催化蒜氨酸分解产生蒜素的......
利用甲醇诱导重组Pichia pastoris在发酵罐中表达几丁质酶，在发酵过程中根据溶氧变化控制甲醇流加速度。结果显示。蛋白表达量随菌......
考察了5L发酵罐中三种甲醇流加策略（恒溶氧、恒比生长速率和恒甲醇浓度）对重组毕赤酵母表达人肝再生增强因子（hALR）的影响。控制恒定甲......
目的在毕赤酵母中表达人纤溶酶原K5基因，并检测hK5蛋白的抗肿瘤活性。方法根据酵母遗传密码的偏爱性优化设计hK5基因，构建重组表达质......
pichiapastoris能使外源真核基因正确翻译和翻译后加工,并能对许多蛋白质产物进行分泌,使产物易于提纯,是一种极具潜力的酵母表达......
利用已有的猪胃蛋白酶原AcDNA，构建毕赤酵pPIC3．5K—pA胞内表达载体．经纤维蛋白消化试验和干酪素琼脂平板法筛选，获得两株高表达的重组......
